Primer Design and Libraries

Designing primers for LAMP and PCR requires careful attention to several factors.  The binding sequences must be complementary only to their binding sites, with an appropriate melting temperature and binding energy.  Primers must not be complementary with one another, and binding sites should be spaced appropriately.  VosBio™ has developed a method for predicting the most efficient primer sets by optimizing several parameters in parallel to identify the best primer candidates.  Our approach can generate primer sets for any length of target sequence, and results may be sorted based on any parameter of interest. 

As we continually refine our process for identifying top primers for a given target, we are building up a library of primer sets suitable for LAMP and PCR amplification of various common respiratory viruses.  Please contact us for primer design assistance.