Enzyme-Linked Immunosorbent Assay (ELISA) is a powerful and sensitive tool for detecting and quantifying specific proteins and biomarkers in biological samples.  When applied to Exhaled Breath Condensate (EBC), ELISA offers a non-invasive approach for assessing airway inflammation and other respiratory conditions by measuring key mediators such as cytokines, chemokines, and leukotrienes.  This method has been successfully used in studies to monitor respiratory diseases like asthma and Chronic Obstructive Pulmonary Disease (COPD), providing valuable insights into disease mechanisms and treatment responses.  As a highly adaptable technique, ELISA continues to demonstrate its potential for expanding diagnostic capabilities in EBC research.